Table 1 Laboratory methods for bacteria identification and susceptibility testing
Organism | Media | Tests for presumptive identification | Susceptibility testing Method |
|---|---|---|---|
Enterobacteriaceae and other Gram-negative bacilli | BAP MAP | Gram stain Colony characteristics Indole test Methyl red test Voges-Proskauer test Citrate utilization test Urease test Triple sugar iron (TSI) test | Disc diffusion |
Haemophilus species | BAP MAP | Gram stain Colony characteristics Hemolysis X and V growth factor requirements | Disc diffusion |
Neisseria species | BAP CAP TMA | Gram stain Colony characteristics Catalase test Oxidase test Growth on Thayer-Martin | Disc diffusion |
Streptococci species | BAP CAP | Gram stain Hemolysis Bile solubility test Optochin susceptibility Bacitracin susceptibility | Disc diffusion |
Staphylococci species | BAP CAP MSA | Gram stain Catalase test Coagulase test Novobiocin susceptibility | Disc diffusion |
Pseudomonas species | BAP MAP | Gram stain Colony characteristics Oxidase test Oxidative fermentation | Disc diffusion |