An outbreak of gastroenteritis associated with a novel GII.8 sapovirus variant-transmitted by vomit in Shenzhen, China, 2019

Table 1 Primers used in this study for sapovirus detection and amplification of almost whole genome

	Primer	Nucleotide sequence (5′-3′)	Position	Source
Amplification of almost complete genome	orf-1	GAGTTGAACACGCAGTCC	42-59^a	This study
	orf-1	ATGATGGCACCAGTAAGG	1009-1026^a
	orf-2	CGCAGTGTCAGTGGTGTC	880-897^a
	orf-2	AAGGTATGGTCGAACGAA	1880-1897^a
	orf-3	CGTCGTGTTCTAACTGTTGA	1763-1782^a
	orf-3	GAATCTTGTGATAACCTCCATA	2740-2761^a
	orf-4	CCAATTAGTAGCTGAAACCCTT	2719-2740^a
	orf-4	CCCTTCCACGCAAACACG	3634-3651^a
	orf-5	CAGAGGGCACTTATGAGAC	3432-3450^a
	orf-5	CAGAGGCAGTTATGGGAG	4434-4451^a
	orf-6	GGGTCGTGTATTGTTTGG	4335-4352^a
	orf-6	CCGAGTTTGGCATTTCTA	5280-5297^a
	orf-7	TAGTGTTTGAAATGGAGGGC	5157-5176^a
	orf-7	TTGGGAAGTGACTGCTGA	6164–6181^a
	orf-8	AGGGCATCATCTTTCCAC	6114–6131^a
	orf-8	TTTGCGAGACAGTCCATTA	7029-7047^a
	orf-9	GGAATCAACTGCGGAAAT	6577-6594^a
	orf-9	GAATAAGACAGCGATGGTC	7429-7447^a
	AP1	CTCGCCACCTATGAAGCA	5081-5098^a
	AP1	AATCGGCAGTGATGTCCT	7004-7021^a
	Orf1–1	CTTCTAAGCCATTCTACTCAA	5-25^b
	Orf1–1	CGTGCCATGAACTGTTTG	1179-1196^b
	Orf1–2	GGCAGCATCACATCAGTC	1093-1110^b
	Orf1–2	TGTTTGCAGACATGAACG	2078-2095^b
	Orf1–1,2	CTTCTAAGCCATTCTACTCAA	5-25^b
	Orf1–1,2	GCTCTGTTTGCAGACATG	6206-6323^b
	Orf1–4	ACCGTGGGTGGTATGACT	2479-2496^b
	Orf1–4	GGCGACAACCGTTGAAAT	3269-3286^b

^aPosition in the complete sapovirus GII genogroup sequence (accession no: MG674584)
^bPosition in the complete sapovirus GII genogroup sequence (accession no: MF462287)

ISSN: 1471-2334