Table 2 Primer sequences, fragment size, and PCR conditions for detection of virulence genes in Pseudomonas aeruginosa
Gene | Primer sequence (5′➔3′) | Fragment size (bp) | Annealing T (°C) |
|---|---|---|---|
algD | F: AAGGCGGAAATGCCATCTCC | 290 | 60 |
R: AGGGAAGTTCCGGGCGTTTG | |||
exoY | F: TATCGACGGTCATCGTCAGGT | 1035 | |
R: TTGATGCACTCGACCAGCAAG | |||
plcH | F: GCACGTGGTCATCCTGATGC | 608 | |
R: TCCGTAGGCGTCGACGTAC | |||
rhIR | F: TGCATTTTATCGATCAGGGC | 133 | 57 |
R: CACTTCCTTTTCCAGGACG | |||
lasI | F: CGTGCTCAAGTGTTCAAGG | 295 | |
R: TACAGTCGGAAAAGCCCAG | |||
apr | F: TGTCCAGCAATTCTCTTGC | 1017 | |
R: CGTTTTCCACGGTGACC | |||
exoT | F: CAATCATCTCAGCAGAACCC | 1159 | |
R: TGTCGTAGAGGATCTCCTG | |||
rhII | F: TTCATCCTCCTTTAGTCTTCCC | 155 | |
R: TTCCAGCGATTCAGAGAGC | |||
exoS | F: CGTCGTGTTCAAGCAGATGGTGCTG | 444 | 65 |
R: CCGAACCGCTTCACCAGGC | |||
plcN | F: TCCGTTATCGCAACCAGCCCTACG | 481 | |
R: TCGCTGTCGAGCAGGTCGAAC | |||
lasB | F: GGAATGAACGAAGCGTTCTCCGAC | 284 | |
R: TGGCGTCGACGAACACCTCG | |||
lasR | F: AAGTGGAAAATTGGAGTGGAG | 130 | |
R: GTAGTTGCCGACGACGATGAAG | |||
algU | F: CGCGAACCGCACCATCGCTC | 1500 | 66 |
R: GCCGCACGTCACGAGC | |||
exoU | F: TACCAGGTACGGCCATGTTC | 575 | 59 |
R: ACGCTCTGAAGCCTGAAGAC |