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Table 3 Comparison of Duplex-PCR and different result combination of other diagnostic methods tested

From: Evaluation of the performance of an in-house duplex PCR assay targeting the IS6110 and rpoB genes for tuberculosis diagnosis in Cameroon

Sample category

  

PCR Results

n

%

IS6110

rpoB

D-PCR

  

(−)

(+)

(−)

(+)

(−)

(+)

Smear (+) Culture (+)

44

34

3

41

4

40

2

42

Smear (−) Culture (+)

7

5.4

1

6

3

4

1

6

Smear (−) Culture (−)

62

48

58

4

58

4

58

4

Smear (+) Culture contaminated

1

0.7

0

1

0

1

0

1

Smear (−) Culture contaminated

5

3.9

5

0

5

0

5

0

Smear (+) Culture (−)

2

1.6

0

2

0

2

0

2

Clinically diagnosed TB

8

6.2

1

7

2

6

1

7

Total

129

100

68

61

72

57

67

62

  1. (−) = negative, (+) = Positive