Table 1 Differences in quantification cycle (Cq) values between pooling and individual testing by Allplex™2019-nCoV assay
Pool size | Parameter | Testing Condition | E gene Mean (range) | N gene Mean (range) | RdRP gene Mean (range) |
|---|---|---|---|---|---|
P20 (n = 26) | Cq value | individual | 24.63 (12.96, > 40) | 27.79 (15.4, > 40) | 26.23 (13.69, 33.45) |
pool | 29.67 (16.48, > 40) | 32.65 (19.9, > 40) | 31.39 (18.2, > 40) | ||
difference | 5.04 (−1.06, 13.15) | 4.86 (−0.42, 11.52) | 5.16 (−1.39, 11.57) | ||
SP5 (n = 14) | Cq value | individual | 21.38 (12.96, 28.48) | 25.92 (15.4, > 40) | 23.34 (13.69, 31.15) |
pool | 24.49 (14.5, > 40) | 28.30 (18.23, > 40) | 26.42 (16.48, > 40) | ||
difference | 3.11 (−2.26, 13.01) | 2.37 (−2.32, 6.66) | 3.08 (−3.33, 10.22) |