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Table 1 Differences in quantification cycle (Cq) values between pooling and individual testing by Allplex™2019-nCoV assay

From: Pooling for SARS-CoV-2 control in care institutions

Pool size Parameter Testing Condition E gene
Mean (range)
N gene
Mean (range)
RdRP gene
Mean (range)
P20 (n = 26) Cq value individual 24.63 (12.96, > 40) 27.79 (15.4, > 40) 26.23 (13.69, 33.45)
pool 29.67 (16.48, > 40) 32.65 (19.9, > 40) 31.39 (18.2, > 40)
difference 5.04 (−1.06, 13.15) 4.86 (−0.42, 11.52) 5.16 (−1.39, 11.57)
SP5 (n = 14) Cq value individual 21.38 (12.96, 28.48) 25.92 (15.4, > 40) 23.34 (13.69, 31.15)
pool 24.49 (14.5, > 40) 28.30 (18.23, > 40) 26.42 (16.48, > 40)
difference 3.11 (−2.26, 13.01) 2.37 (−2.32, 6.66) 3.08 (−3.33, 10.22)
  1. P20 Pool of 20 samples, SP5 sub pool of 5 samples, Cq Quantification cycle