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Table 4 Analysis of the test of neutrality (Tajima’s D and Fu and Li′s D and F test statistic) at Pvmdr-1, Pvdhr, Pvdhps

From: Distribution pattern of amino acid mutations in chloroquine and antifolate drug resistance associated genes in complicated and uncomplicated Plasmodium vivax isolates from Chandigarh, North India

 

Occurrence of mutation

H

Hd (±SD)

Nucleotide Diversity

Tajima’s D

Fu and Li′s D and F test statistic

Pvmdr-1

n (%)

  

π (±SD)

  

Complicated

41 (100)

2

0.048 ± 0.045

0.00009 ± 0.00009

−1.11966

−1.78899; − 1.84602

Uncomplicated

77 (100)

4

0.081 ± 0.044

0.00015 ± 0.00008

−1.65144

−3.24282*; −3.21499*

Combined

118 (100)

3

0.034 ± 0.023

0.00007 ± 0.00005

−1.35708

−2.88867*; −2.82660*

Pvdhfr

Complicated

11 (22.9)

6

0.713 ± 0.035

0.00228 ± 0.00024

−0.18017

−0.30060; −0.30785

Uncomplicated

17 (20)

3

0.614 ± 0.033

0.00165 ± 0.00016

1.38695

0.84089; 1.18451

Combined

28 (21)

5

0.652 ± 0.019

0.00182 ± 0.00012

0.51341

−1.18432; −0.73304

Pvdhps

Complicated

7 (15.5)

3

0.341 ± 0.081

0.00064 ± 0.00016

−0.40771

0.75830;0.48349

Uncomplicated

4 (5)

3

0.164 ± 0.054

0.00031 ± 0.00010

−0.94808

−1.01710; −1.16199

Combined

11 (9)

3

0.229 ± 0.047

0.00044 ± 0.00016

−0.99655

−0.63719; − 0.63719

  1. S = Number of polymorphic (segregating) sites
  2. π = observed average pair-wise nucleotide diversity
  3. Rm = Minimum number of recombination events
  4. H = number of nucleotide haplotypes
  5. Hd = haplotype diversity
  6. * = p < 0.05