Skip to main content

Table 2 Comparison of sensitivities of the long-range RT-qPCR with the conventional RT-qPCR and the plaque assay

From: Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

 

RT-qPCR (copies/ml)

Normalized to plaque assay (%)

PB2

5.7 × 107

261

PA

5.4 × 106

24

HA

5.3 × 108

2395

NA

6.8 × 108

3109

NP

4.5 × 108

2041

M

3.6 × 108

1627

NS

1.5 × 108

700

Conventional RT-qPCR

9.6 × 109

43,636

  1. Original copy numbers measured in the RT-qPCRs were divided by that in the conventional RT-qPCR or the titer in the plaque assay. Values are shown as percentages. PB2, PA, HA, NA, NP, M, and NS indicates the long-range RT-qPCR for PB2, PA, HA, NA, NP, M, and NS, respectively. Normalization was performed as follows: RT-qPCR (copies/ml) / Plaque assay (2.2 × 107 pfu/ml)
Back to article page

BMC Infectious Diseases

ISSN: 1471-2334

Contact us