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Table 2 Primers for PCR of virulence and superantigen genes

From: Molecular epidemiology and antimicrobial resistance of group a streptococcus recovered from patients in Beijing, China

Gene Primer direction Primer
sequence
Annealing
Temperature (°C)
Amplicon
Size (bp)
speA Forward 5′-ATGGAAAACAATAAAAAAGTATTG-3’ 52 756
  Reverse 5′-TTACTTGGTTGTTAGGTAGACTTC-3’   
spec Forward 5′-AATTTCGATTCTGCCGCTTA-3’ 52 400
  Reverse 5′-GCAGGGTAAATTTTTCAACGAC-3’   
speG Forward 5′-TCATGTGTTTTTAGCTATGGAAGTC-3’ 52 590
  Reverse 5′- ACTGTCTCGACTTAAAAGCTTATCA-3’   
speH Forward 5′-AGATTGGATATCACAGG-3’ 52 416
  Reverse 5′- CTATTCTCTCGTTATTGG-3’   
speI Forward 5′- AATGAAGGTCCGCCATTTTC-3’ 52 516
  Reverse 5′-TCTCTCTGTCACCATGTCCTG-3’   
speJ Forward 5′-GATAGTGAAAATATTAAAGACG-3’ 52 630
  Reverse 5′- TTATTTAGTCCAAAGGTAAATATC-3’   
speK Forward 5′-GTGTGTCTAATGCCACCGTCT-3’ 52 564
  Reverse 5′-GGAACATATATGCTCCTAGAT-3’   
speL Forward 5′-CAGCACCTTCCTCTTTCTCG-3’ 52 459
  Reverse 5′-GGAAAAAGAGGGACGCAAG-3’   
speM Forward 5′-GGATGAGTGAATAAATCGGTAAAC-3’ 55 425
  Reverse 5′-AGTCTGGGACGATGATAA-3’   
ssa Forward 5′-TGATCAAATATTGCTCCAGGTG-3’ 52 502
  Reverse 5′-TCCACAGGTCAGCTTTTACAG-3’   
smeZ Forward 5′-CTTCAATATTCATTGCAATAATTTC-3’ 52 430
  Reverse 5′-TGTAACTGTTGTTTTGTTAGTTGAT-3’   
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