Table 2 Primers for PCR of virulence and superantigen genes
Gene | Primer direction | Primer sequence | Annealing Temperature (°C) | Amplicon Size (bp) |
|---|---|---|---|---|
speA | Forward | 5′-ATGGAAAACAATAAAAAAGTATTG-3’ | 52 | 756 |
|  | Reverse | 5′-TTACTTGGTTGTTAGGTAGACTTC-3’ |  |  |
spec | Forward | 5′-AATTTCGATTCTGCCGCTTA-3’ | 52 | 400 |
|  | Reverse | 5′-GCAGGGTAAATTTTTCAACGAC-3’ |  |  |
speG | Forward | 5′-TCATGTGTTTTTAGCTATGGAAGTC-3’ | 52 | 590 |
|  | Reverse | 5′- ACTGTCTCGACTTAAAAGCTTATCA-3’ |  |  |
speH | Forward | 5′-AGATTGGATATCACAGG-3’ | 52 | 416 |
|  | Reverse | 5′- CTATTCTCTCGTTATTGG-3’ |  |  |
speI | Forward | 5′- AATGAAGGTCCGCCATTTTC-3’ | 52 | 516 |
|  | Reverse | 5′-TCTCTCTGTCACCATGTCCTG-3’ |  |  |
speJ | Forward | 5′-GATAGTGAAAATATTAAAGACG-3’ | 52 | 630 |
|  | Reverse | 5′- TTATTTAGTCCAAAGGTAAATATC-3’ |  |  |
speK | Forward | 5′-GTGTGTCTAATGCCACCGTCT-3’ | 52 | 564 |
|  | Reverse | 5′-GGAACATATATGCTCCTAGAT-3’ |  |  |
speL | Forward | 5′-CAGCACCTTCCTCTTTCTCG-3’ | 52 | 459 |
|  | Reverse | 5′-GGAAAAAGAGGGACGCAAG-3’ |  |  |
speM | Forward | 5′-GGATGAGTGAATAAATCGGTAAAC-3’ | 55 | 425 |
|  | Reverse | 5′-AGTCTGGGACGATGATAA-3’ |  |  |
ssa | Forward | 5′-TGATCAAATATTGCTCCAGGTG-3’ | 52 | 502 |
|  | Reverse | 5′-TCCACAGGTCAGCTTTTACAG-3’ |  |  |
smeZ | Forward | 5′-CTTCAATATTCATTGCAATAATTTC-3’ | 52 | 430 |
|  | Reverse | 5′-TGTAACTGTTGTTTTGTTAGTTGAT-3’ |  |  |