Table 4 Correlation between Real-Time PCR T86I mutation MICs values of ciprofloxacin and mutations of the gyrA gene
Real Time PCR Mutation T86I | Strains (n = 100) | CIP_MIC (μg/mL) | T86I | A120V | D144N | V149I | H81 | G110 | S119 | Y152 | S157 | V161 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
ACA | GCC | GAT | GTT | CAC | GGC | AGT | TAT | AGC | GTT | |||
ATA | GTC | AAT | ATT | CAT | GGT | AGC | TAC | AGT | GTC | |||
Positive | 28 | 12–32 | T | T | – | – | T | – | C | – | T | C |
Positive | 28 | 12–32 | T | T | – | A | T | – | C | – | T | C |
Positive | 7 | 32 | T | T | – | – | T | T | C | – | T | C |
Positive | 3 | 12–32 | T | T | – | – | T | – | C | C | T | C |
Positive | 6 | 6–32 | T | – | – | – | – | – | – | – | – | – |
Negative | 21 | 0.016–0.190 | – | T | – | – | T | – | C | – | T | C |
Negative | 1 | 0.064 | – | T | – | – | T | T | C | – | T | C |
Negative | 1 | 0.032 | – | T | A | – | T | – | C | – | T | C |
Negative | 5 | 0.023–0.047 | – | – | – | – | – | – | – | – | – | – |