Table 1 Nested PCR (nPCR) conditions as well as oligonucleotide primers used in this study
Target genea | Primer name (sequences; 5′ → 3′) | Amplicon size (bp) |
|---|---|---|
groEL nPCR for Anaplasmataceae (external primers) | GRO607F (GAAGATGCWGTWGGWTGTACKGC) GRO1294R (AGMGCTTCWCCTTCWACRTCYTC) | 539 |
groEL nPCR for Anaplasmataceae (internal primers) | GRO677F (ATTACTCAGAGTGCTTCTCARTG) GRO1121R (TGCATACCRTCAGTYTTTTCAAC) | 450 |
16S rRNA nPCR for Anaplasma and Ehrlichia species (external primers) | AE1-F (AAGCTTAACACATGCAAGTCGAA) AE1-R (AGTCACTGA CCCAACCTTAAATG) | 1406 |
16S rRNA nPCR for A. phagocytophilum (internal primers) | AP-F (GTCGAACGGATTATTCTTTATAGCTTGC) AP-R (CCCTTCCGTTAAGAAGGATCTAATCTCC) | 926 |
ankA nPCR for A. phagocytophilum (external primers) | ANK-F1 (GAAGAAATTACAACTCCTGAAG) ANK-R1 (CAGCCAGATGCAGTAACGTG) | 705 |
ankA nPCR for A. phagocytophilum (internal primers) | ANK-F2 (TTGACCGCTGAAGCACTAAC) ANK-R2 (ACCATTTGCTTCTTGAGGAG) | 664 |
sca1 nPCR for SFG Rickettsia (external primers) | sca1-6545F (ATTCGTAACAGATTAGATRC) sca1-7360R (TTATAGGATGTTTTCGGTTG) | 815 |
sca1 nPCR for SFG Rickettsia (internal primers) | sca1-6647F (TGGATGCGTGSTATGTACG) sca1-7354R (GATGTTTTCGGTTGYTTCGG) | 707 |
ompA nPCR for SFG Rickettsia (external primers) | R190.70F (ATGGCGAATATTTCTCCAAAAA) RR190.701R (GTTCCGTTAATGGCAGCATCT) | 634 |
ompA nPCR for SFG Rickettsia (internal primers) | R190.70F (ATGGCGAATATTTCTCCAAAAA) RR190.602R (AGTGCAGCATTCGCTCCCCCT) | 535 |