Table 5 Results of examination the minimal incubation time required for the LAMP assay when various concentrations of DNA template have been used
Incubation time (min) | DNA dilution (used as a template) | Negative control | ||||
|---|---|---|---|---|---|---|
1.42 ng/μl | 142 pg/μl | 14.2 pg/μl | 1.42 pg/μl | 142 fg/μl | ||
10 | – | – | – | – | – | – |
20 | – | – | – | – | – | – |
30 | + | – | – | – | – | – |
40 | + | + | – | – | – | – |
50 | + | + | + | – | – | – |
60 | + | + | + | + | – | – |