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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Unusual accumulation of a wide array of antimicrobial resistance mechanisms in a patient with cytomegalovirus-associated hemophagocytic lymphohistiocytosis: a case report

Fig. 2

Genotype and antiviral resistance profiles of the cytomegalovirus strain. Nucleic acid extract from patient serum was subjected to NGS library preparation using Nextera XT kit (Illumina, USA) and sequencing was performed on a MiSeq (Illumina). Paired sequence reads were mapped to UL97 and UL54 sequences (Gene ID 3077517 and GenBank accession ABV71585.1, respectively) to obtain corresponding gene sequences from the patient’s CMV strain. The sequences were then analyzed by using an online mutational resistance analyzer (MRA) available from the University of Ulm, https://www.informatik.uni-ulm.de/ni/mitarbeiter/HKestler/mra/app/index.php?plugin=form [5]. Ganciclovir resistance was confirmed by the presence of the A594V mutation in UL97 [6]

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BMC Infectious Diseases

ISSN: 1471-2334

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