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Table 2 Flow cytometry-based detection (%) of flavivirus envelope protein in subpopulations of human peripheral blood mononuclear cells after ex-vivo infectiona

From: Zika virus infects human blood mononuclear cells

Donors

PBMC subpopulations (%)

CD3+CD4+4G2+

CD3+CD8+4G2+

CD3CD19+4G2+

CD14+4G2+

D1

29.5

26.9

17.0

68.1

D3

47.3

40.6

47.3

zero

D4

0.6

zero

50.7

NDb

D5

1.4

1.1

4.0

NDb

D7

0.6

zero

zero

zero

D9

zero

zero

zero

3.9

D14

zero

zero

zero

0.7

D15

zero

zero

1.2

zero

D16

zero

1.7

zero

zero

  1. aInfection rate was calculated by subtracting the percentage of 4G2+ cells in PBMCs who received ZIKV as compared with the background 4G2 labeling profile in PBMCs from the same donor, but that were not exposed to ZIKV (MOCK). Donors D2, D6, D8, D10, D11, D12 and D13 cells were all negative for 4G2 staining. bND = not determined, as very low amounts of these subpopulations were detected