Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

Li, Xuehan; Huang, Tao; Xu, Kai; Li, Chenglin; Li, Yirong

doi:10.1186/s12879-019-4547-5

BMC Infectious Diseases

Table 1 Primers used in this study, and the results of SCCmec types I–V

From: Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

Primer	Nucleotide sequence(5′-3′)	Target gene	Amplicon size(bp)	SCCmec type
Primer	Nucleotide sequence(5′-3′)	Target gene	Amplicon size(bp)	I	II	III	IV	IVa	IVb	IVc	IVd	V
β	ATTGCCTTGATAATAGCCYTCT	ccrA2-B	937		X		X
a3	TAAAGGCATCAATGCACAAACACT
ccrCF	CGTCTATTACAAGATGTTAAGGATAAT	ccrC	518			X						X
ccrCR	CCTTTATAGACTGGATTATTCAAAATAT
1272F1	GCCACTCATAACATATGGAA	IS1272	415	X			X
1272R1	CATCCGAGTGAAACCCAAA
5RmecA	TATACCAAACCCGACAACTAC	mecA–IS431	359									X
5R431	CGGCTACAGTGATAACATCC
Type IVa-F	GCCTTATTCGAAGAAACCG	–	776					X
Type IVa-R	CTACTCTTCTGAAAAGCGTCG
Type IVb-F	TCTGGAATTACTTCAGCTGC	–	493						X
Type IVb-R	AAACAATATTGCTCTCCCTC
Type IVc-F	ACAATATTTGTATTATCGGAGAGC	–	200							X
Type IVc-R	TTGGTATGAGGTATTGCTGG
Type IVd-F	CTCAAAATACGGACCCCAATACA	–	881								X
Type IVd-R	TGCTCCAGTAATTGCTAAAG
Spa-1113f	TAAAGACGATCCTTCGGTGAGC	spa	–
Spa-1514r	CAGCAGTAGTGCCGTTTGCTT
arcC-F	TTGATTCACCAGCGCGTATTGTC	arcC	456
arcC-R	AGG TATCTGCTTCAATCAGCG
aroE-F	ATCGGAAATCCTATTTCACATTC	aroE	456
aroE-R	GGTGTTGTATTAATAACGATATC
glpF-F	CTAGGAACTGCAATCTTAATCC	glpF	465
glpF-R	TGGTAAAATCGCATGTCCAATTC
gmk-F	ATCGTTTTATCGGGACCATC	gmk	417
gmk-R	TCATTAACTACAACGTAATCGTA
pta-F	GTTAAAATCGTATTACCTGAAGG	pta	474
pta-R	GACCCTTTTGTTGAAAAGCTTAA
tpi-F	TCGTTCATTCTGAACGTCGTGAA	tpi	402
tpi-R	TTTGCACCTTCTAACAATTGTAC
yqiL-F	CAGCATACAGGACACCTATTGGC	yqiL	516
yqiL-R	CGTTGAGGAATCGATACTGGAAC
PVL-F	ATCATTAGGTAAAATGTCTGGACATGATCCA	pvl	433
PVL-R	GCATCAASTGTATTGGATAGCAAAAGC
FnbA-F	GTGAAGTTTTAGAAGGTGGAAAGATTAG	fnbA	643
FnbA-R	GCTCTTGTAAGACCATTTTTCTTCAC
FnbB-F	GTAACAGCTAATGGTCGAATTGATACT	fnbB	524
FnbB-R	CAAGTTCGATAGGAGTACTATGTTC
Hla-F	CTGATTACTATCCAAGAAATTCGATTG	hla	209
Hla-R	CTTTCCAGCCTACTTTTTTATCAGT
Hlb-F	GTGCACTTACTGACAATAGTGC	hlb	309
Hlb-R	GTTGATGAGTAGCTACCTTCAGT
Sea-F	GAAAAAAGTCTGAATTGCAGGGAACA	sea	560
Sea-R	CAAATAAATCGTAATTAACCGAAGGTTC
Seb-F	ATTCTATTAAGGACACTAAGTTAGGGA	seb	404
Seb-R	ATCCCGTTTCATAAGGCGAGT
Sec-F	GTAAAGTTACAGGTGGCAAAACTTG	sec	297
Sec-R	CATATCATACCAAAAAGTATTGCCGT
eta-F	CGCTGCGGACATTCCTACATGG	eta	676
eta-R	TACATGCCCGCCACTTGCTTGT
etb-F	CAGATAAAGAGCTTTATACACACATTAC	etb	612
etb-R	AGTGAACTTATCTTTCTATTGAAAAACACTC
clfA-F	ATTGGCGTGGCTTCAGTGCT	clfa	292
clfA-R	CGTTTCTTCCGTAGTTGCATTTG
ermA-F	GTTCAAGAAC AATCAATACA GAG	ermA	421
ermA-R	GGATCAGGAA AAGGACATTT TAC
ermB-F	CCGTTTACGA AATTGGAACA GGTAAAGGGC	ermB	359
ermB-R	GAATCGAGAC TTGAGTGTGC
ermC-F	GCTAATATTG TTTAAATCGT CAATTCC	ermC	572
ermC-R	GGATCAGGAA AAGGACATTT TAC
tetM-F	AGTGGAGCGATTACAGAA	tetM	158
tetM-R	CATATGTCCTGGCGTGTCTA
tetK-F	GTAGCGACAATAGGTAATAGT	tetK	360
tetK-R	GTAGTGACAATAAACCTCCTA
tetL-F	ATAAATTGTTTCGGGTCGGTAAT	tetL	1077
tetL-R	AACCAGCCAACTAATGACAATGAT
tetO-F	AACTTAGGCATTCTGGCTCAC	tetO	514
tetO-R	TCCCACTGTTCCATATCGTCA

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ISSN: 1471-2334

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