Reference condition | Test condition | Conclusion | |
---|---|---|---|
% of positive calls | % of positive calls | ΔCt | |
< 85% | Any % | Any ΔCt | The experiment is invalid. The causes of the shift in sensitivity must be investigated and the experiment reconducted. |
≥ 85% | < 85% | Any ΔCt | The highly-concentrated DNA or the tested substance is considered to be interfering with the detection of the target pathogen if any of these 2 criteria are met. |
Any % | ΔCt ≥ 2SDrep | ||
≥ 85% | ≥ 85% | Any ΔCt | The highly-concentrated DNA or the tested substance is considered not to be interfering with the detection of the target pathogen if both criteria are met. |
Any % | ΔCt < 2SDrep |