Table 2 Stimuli and immune response of TruCulture®

1

Heat killed Candida albicans

Whole microbe providing complex immune response, including activation of TLR6

2

Lipopolysaccharide from E.coli (LPS)

Bacterial endotoxin. Activator of TLR4.

3

Resiquimod R848

Synthetic agonist of TLR7 and TLR8 (both responding to single-stranded RNA)

4

Polyinosinic:polycytidylic acid (Poly I:C)

Analogue of double-stranded RNA. Activator of TLR3

5

None (negative control)

Allows for assessment of in vivo activation