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Table 1 Components of PCR master mix

From: A highly sensitive modified nested PCR to enhance case detection in leishmaniasis

PCR water 19.8 μl
2XPCR Taq master mix (abm, G013) 25.0 μl
Forward, outer primer (P221) 0.1 μl
Reverse, outer primer (P332) 0.1 μl
Template DNA 5.0 μl
Total 50.0 μl