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Table 1 Primers and PCR amplification conditions used for molecular identification

From: The first case report of kerion-type scalp mycosis caused by Aspergillus protuberus

Locus Primers Primer sequence Amplification conditions
ITS ITS5 5′-GGAAGTAAAAGTCGTAACAAGG-3′ 94 °C 5 min; 30 cycles: 94 °C 60s, 55 °C 60s, 72 °C 60s; 72 °C 10 min
ITS4 5′-TCCTCCGCTTATTGATATGC-3′
BT2 BT2a 5′-GGTAACCAAATCGGTGCTGCTTTC-3′ 95 °C 5 min; 35 cycles: 95 °C 30s, 58 °C 30s, 72 °C 1 min; 72 °C 7 min
BT2b 5′-ACCCTCAGTGTAGTGACCCTTGGC-3′