Table 1 Primers and PCR amplification conditions used for molecular identification
From: The first case report of kerion-type scalp mycosis caused by Aspergillus protuberus
Locus | Primers | Primer sequence | Amplification conditions |
|---|---|---|---|
ITS | ITS5 | 5′-GGAAGTAAAAGTCGTAACAAGG-3′ | 94 °C 5 min; 30 cycles: 94 °C 60s, 55 °C 60s, 72 °C 60s; 72 °C 10 min |
ITS4 | 5′-TCCTCCGCTTATTGATATGC-3′ | ||
BT2 | BT2a | 5′-GGTAACCAAATCGGTGCTGCTTTC-3′ | 95 °C 5 min; 35 cycles: 95 °C 30s, 58 °C 30s, 72 °C 1 min; 72 °C 7 min |
BT2b | 5′-ACCCTCAGTGTAGTGACCCTTGGC-3′ |