Table 2 PCR primers and Luminex probes in this study. All reverse primers are 5′- biotinylated. All probes have 5′-amino-C12-modified. P, AEGIS nucleotide; *, SAMRS nucleotides. R, mixed A, and G bases. Position of primers and probes refer to the chosen sequence of the viral strains

DENV1 Forward primer

GGCCRGATTAAGCC*A*T*A*G

KY926849.1

UTR

DENV1 Probe

APAPCTATPCTPCCTPT

DENV1 Reverse primer

GCTTTCGGCCTGA*C*T*T*C

DENV2 Forward primer

CGTGTCRACTGTRCA*A*C*A*G

KY461768.1

Capsid/pre-membrane protein gene

DENV2 Probe

ATTCTCACTTPPAATPCTPC

DENV2 Reverse primer

ARTATCCCTGCTGTT*G*G*T*G

DENV3 Forward primer

AACACTCTGGGAAGGAT*C*A*C*C

KT726361.1

Non-structural Protein NS4B

DENV3 Probe

TTPPAACACCACPATAPCT

DENV3 Reverse primer

AGCAAGCCCAGCT*C*C*T*G

DENV4 Forward primer

GCAGGCAAAAGCCA*C*A*A*G

AH0119363.2

Non-structural protein NS2A

DENV4 Probe

APTPPACPPPATAACAPT

DENV4 Reverse primer

CATGACCTGCCCTA*A*T*T*G

CHIKV Forward primer

CAGATGGCAACGAA*C*A*G*G

KY575571.1

Non-structural protein nsP4

CHIKV FS-1 Probe

CCTTTPCAAPCTCCAPATC

CHIKV Reverse primer

GGGTCCTCTGAGCT*T*C*T*C

ZIKV Forward primer

AGGGACCTCCGACT*G*A*T*G

KY415991.1

Non-structural protein NS5

ZIKV Probe

PAAAPPPAPAATPPATPACC

ZIKV Reverse primer

CCTCAATCCACACTCT*G*T*T*C