Fig. 2

The six-fold multiplexed xMAP Luminex diagnostics panel for detection of DENV1–4, CHIKV, and ZIKV in mosquitoes was developed using viral RNAs (Table 1). Luminex DHA profiles are presented: each DHA was performed with biotinylated viral PCR-amplicon and six target-specific, dPTP-containing probes. The SAMRS technology was employed for RT-PCR, and AEGIS technology for molecular hybridization on Luminex beads. MFI, median fluorescent intensity (Luminex unit); Background, sample buffer was added to the wells; NTC, no template control, ddH₂0 was added to RT-PCR mixture; lower right panel presents Luminex bead identities. Each bead annealed to a target-specific probe: for example, D1 (6–2) #17 stands for DENV1 (D1) probe (6–2) annealed to Luminex bead #17