Fig. 1

Application of advanced fragment analysis (AFA) in screening of encephalitis related pathogens from cerebrospinal fluid (CSF). 300 μl cerebrospinal fluids were used for fragment analysis. After multiplex PCR, the fragments with particular size were separated with capillary electrophoresis, and each fragment of a specific size represents a specific bacterial or virus infection of interest. a Hi-Di size maker determines the size of fragments by conducting capillary electrophoresis together with samples. b The capillary electrophoresis peaks of representative positive control sample. Different sizes of PCR fragments represent different pathogens