Table 1 Primers used for the detection of β-lactamase-encoding genes
| PCR | ß-lactamase genes | Primers | Nucleotide sequences | Amplicon size (bp) |
|---|---|---|---|---|
| Multiplex I | TEM including TEM-1 and TEM-2 |
MultiTSO-T_for MultiTSO-T_rev |
CATTTCCGTGTCGCCCTTATTC CGTTCATCCATAGTTGCCTGAC | 800 |
| SHV including SHV-1 |
MultiTSO-S_for MultiTSO-S_rev |
AGCCGCTTGAGCAAATTAAAC ATCCCGCAGATAAATCACCAC | 713 | |
| OXA-1, OXA-4 and OXA-30 |
MultiTSO-O_for MultiTSO-O_re |
GGCACCAGATTCAACTTTCAAG GACCCCAAGTTTCCTGTAAGTG | 564 | |
| Multiplex II | CTX-M-1, CTX-M-3 and CTX-M-15 |
MultiCTXMGp1_for MultiCTXMGp1-2_rev |
TTAGGAARTGTGCCGCTGYA CGATATCGTTGGTGGTRCCAT | 688 |
| CTX-M-2 |
MultiCTXMGp2_for MultiCTXMGp1-2_re |
CGTTAACGGCACGATGAC CGATATCGTTGGTGGTRCCAT | 404 | |
| CTX-M-9 and CTX-M14 |
MultiCTXMGp9_for MultiCTXMGp9_rev |
TCAAGCCTGCCGATCTGGT TGATTCTCGCCGCTGAAG | 561 | |
| CTX-M-8/25 | CTX-M-8/25 |
CTX-Mg8/25_for CTX-Mg8/25_re |
AACRCRCAGACGCTCTAC TCGAGCCGGAASGTGTYAT | 326 |