Table 1 Primers used for the detection of β-lactamase-encoding genes
PCR | ß-lactamase genes | Primers | Nucleotide sequences | Amplicon size (bp) |
|---|---|---|---|---|
Multiplex I | TEM including TEM-1 and TEM-2 | MultiTSO-T_for MultiTSO-T_rev | CATTTCCGTGTCGCCCTTATTC CGTTCATCCATAGTTGCCTGAC | 800 |
SHV including SHV-1 | MultiTSO-S_for MultiTSO-S_rev | AGCCGCTTGAGCAAATTAAAC ATCCCGCAGATAAATCACCAC | 713 | |
OXA-1, OXA-4 and OXA-30 | MultiTSO-O_for MultiTSO-O_re | GGCACCAGATTCAACTTTCAAG GACCCCAAGTTTCCTGTAAGTG | 564 | |
Multiplex II | CTX-M-1, CTX-M-3 and CTX-M-15 | MultiCTXMGp1_for MultiCTXMGp1-2_rev | TTAGGAARTGTGCCGCTGYA CGATATCGTTGGTGGTRCCAT | 688 |
CTX-M-2 | MultiCTXMGp2_for MultiCTXMGp1-2_re | CGTTAACGGCACGATGAC CGATATCGTTGGTGGTRCCAT | 404 | |
CTX-M-9 and CTX-M14 | MultiCTXMGp9_for MultiCTXMGp9_rev | TCAAGCCTGCCGATCTGGT TGATTCTCGCCGCTGAAG | 561 | |
CTX-M-8/25 | CTX-M-8/25 | CTX-Mg8/25_for CTX-Mg8/25_re | AACRCRCAGACGCTCTAC TCGAGCCGGAASGTGTYAT | 326 |