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Table 2 Calculated the detection limit of LF-RPA assay

From: Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid

DNA amount (pg/reaction) NC 12.8 1.28 0.64 0.32 0.16 0.128 PC
C. neoformans 0/5a 5/5a 5/5a 5/5a 3/5a 1/5a 0/5a 5/5a
DNA amount (pg/reaction) NC 20.32 2.032 1.016 0.508 0.254 0.2032 PC
C. gattii 0/5a 5/5a 5/5a 5/5a 4/5a 1/5a 0/5a 5/5a
  1. aThe number of positive samples/the total number of samples tested by LF-RPA assay. NC: negative control; PC: positive control.