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Table 2 Calculated the detection limit of LF-RPA assay

From: Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid

DNA amount (pg/reaction)

NC

12.8

1.28

0.64

0.32

0.16

0.128

PC

C. neoformans

0/5a

5/5a

5/5a

5/5a

3/5a

1/5a

0/5a

5/5a

DNA amount (pg/reaction)

NC

20.32

2.032

1.016

0.508

0.254

0.2032

PC

C. gattii

0/5a

5/5a

5/5a

5/5a

4/5a

1/5a

0/5a

5/5a

  1. aThe number of positive samples/the total number of samples tested by LF-RPA assay. NC: negative control; PC: positive control.