Fig. 1From: Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation studyEffect of DNase I treatment on P. aeruginosa quantification by qPCR. The effect of DNase I treatment was assessed by comparing the qPCR counts of two aliquots of the same sample, one digested and one undigested. L/D-BC: P. aeruginosa ATCC 9027 broth culture containing live and dead cells. L/D-SP: sputum sample spiked with 3 × 106 cells of a broth culture containing live and dead cells. Log-SP: sputum sample spiked with 3 × 106 cells of a log phase cultureBack to article page