Fig. 3

Functional consequences of HTNV-infection in human podocytes. a Podocytes were infected with HTNV at an MOI of 0.5 and viability was assessed on day six post infection. Control cells remained uninfected and viability was set to 100%. Three independent experiments were performed in triplicates. Shown is mean ± SD. b Motility of uninfected and infected podocytes was analyzed by single cell tracking via live cell imaging. Three independent experiments were performed. In each experiment 30 cells were analyzed. Shown is mean ± SD. c Podocytes seeded into μ-plate wells were infected with HTNV and analyzed by migration assay after six days after infection. After removal of the insert and after eight hours, cell-free areas were measured and relative migration was calculated. Migration of uninfected podocytes was set to 100%. Representative images are shown. Cells were imaged at a magnification of × 100. Three independent experiments were performed. Shown is mean ± SD. d Uninfected and HTNV-infected cells were plated into 96 wells and numbers of attached cells were quantified after one hour. Results were obtained from three independent experiments, each performed in quadruplicate. N protein was stained in red. Cells were imaged at a magnification of × 200. Adhesion of uninfected cells was set to 100%. Shown is mean ± SD