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Table 2 Analysis of replacement (R) and silent (S) mutations in heavy and light chain variable regions of single plasmablast-derived mAbs

From: Human monoclonal antibodies isolated from a primary pneumococcal conjugate Vaccinee demonstrates the expansion of an antigen-driven Hypermutated memory B cell response

Antibody ID Number of clones VH     VL    
   Total NT substitution (%) R/S ratio in Frame work R/S ratio in CDR regions Strong Selection On HCDR Total NT substitution (%) R/S ratio in Frame work R/S ratio in CDR regions Strong Selection on LCDR
1A2 1 6.08% 4/2 10/2 Yes 0.34% 0/0 1/0 No
1A4 1 6.08% 3 /4 11/0 Yes 2.05% 1/0 4/1 Yes
1A6 6 8.59% 7/2 15/1 Yes 3.97% 2/1 8/1 Yes
1A10 1 6.46% 5/5 7/2 Yes 2.72% 0/1 7/0 Yes
1B1 1 1.36% 2/1 1/0 No 1.04% 0/0 3/0 No
1B2 2 4.42% 2/2 8/1 Yes 2.82% 3/0 4/0 Yes
1C3 1 5.78% 4/2 11/0 Yes 2.03% 1/1 4/0 Yes
1C4 2 5.48% 2/1 12/1 Yes 2.38% 5/2 0/0 No
1C6 1 3.75% 1 /2 8/0 Yes 2.37% 0/1 5/1 Yes
1D4 1 6.16% 5/4 9/0 Yes 3.64% 0/3 8/0 Yes
1D7 1 7.43% 5/3 14/0 Yes 2.74% 0/1 6/1 Yes
  1. Note: Antigen-driven selection on HCDR and LCDR regions was judged based on somatic mutation analysis method developed by Shlomchik M et al. [27]