Table 2 Analysis of replacement (R) and silent (S) mutations in heavy and light chain variable regions of single plasmablast-derived mAbs
Antibody ID | Number of clones | VH | Â | Â | Â | VL | Â | Â | Â |
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Total NT substitution (%) | R/S ratio in Frame work | R/S ratio in CDR regions | Strong Selection On HCDR | Total NT substitution (%) | R/S ratio in Frame work | R/S ratio in CDR regions | Strong Selection on LCDR |
1A2 | 1 | 6.08% | 4/2 | 10/2 | Yes | 0.34% | 0/0 | 1/0 | No |
1A4 | 1 | 6.08% | 3 /4 | 11/0 | Yes | 2.05% | 1/0 | 4/1 | Yes |
1A6 | 6 | 8.59% | 7/2 | 15/1 | Yes | 3.97% | 2/1 | 8/1 | Yes |
1A10 | 1 | 6.46% | 5/5 | 7/2 | Yes | 2.72% | 0/1 | 7/0 | Yes |
1B1 | 1 | 1.36% | 2/1 | 1/0 | No | 1.04% | 0/0 | 3/0 | No |
1B2 | 2 | 4.42% | 2/2 | 8/1 | Yes | 2.82% | 3/0 | 4/0 | Yes |
1C3 | 1 | 5.78% | 4/2 | 11/0 | Yes | 2.03% | 1/1 | 4/0 | Yes |
1C4 | 2 | 5.48% | 2/1 | 12/1 | Yes | 2.38% | 5/2 | 0/0 | No |
1C6 | 1 | 3.75% | 1 /2 | 8/0 | Yes | 2.37% | 0/1 | 5/1 | Yes |
1D4 | 1 | 6.16% | 5/4 | 9/0 | Yes | 3.64% | 0/3 | 8/0 | Yes |
1D7 | 1 | 7.43% | 5/3 | 14/0 | Yes | 2.74% | 0/1 | 6/1 | Yes |