Fig. 2
From: Rapid and sensitive detection of Yersinia pestis by lateral-flow assay in simulated clinical samples
a. Superose 6 column(upper) and Q-Sepharose column (lower) purified F1 protein. P1 and P2 fractions were subsequently pooled together as immunized antigen. b: SDS-PAGE of E. coli-derived recombinant F1 protein purified from Q-Sepharose column. c: Western blot assay of E. coli-derived recombinant F1 probed with anti-F1 mouse serum. 1st Ab: Anti-F1 Mab (YPF19); 2nd Ab: AP-conjugated anti-mouse IgG