Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance

Table 2 The influenza virus strains used in the present study

Types and subtypes	Reference strains	GISAID NO.	HP/LP AIV	292R/K in NA	Virus titer (TCID₅₀/mL)	qRT-PCR assays
Types and subtypes	Reference strains	GISAID NO.	HP/LP AIV	292R/K in NA	Virus titer (TCID₅₀/mL)	H7-W	H7-M	N9-W	N9-M
H7N9	SZ/Th003	EPI_ISL_250313	LP	R	2 × 10⁶	14.38 ± 0.12	U^c	14.44 ± 0.21	U
H7N9	SZ/Th004	EPI_ISL_250314	LP	K	2 × 10⁶	14.37 ± 0.36	U	15.05 ± 0.23	14.15 ± 0.55
H7N9	GD/Th005	EPI_ISL_250312	HP	R	2 × 10⁶	15.61 ± 0.48	15.06 ± 0.49	15.45 ± 0.19	U
H7N9	SZ/Th007	EPI_ISL_259757	HP	K	2 × 10⁶	13.91 ± 0.24	13.87 ± 0.56	14.45 ± 0.15	13.55 ± 0.09
H3N2	CAS0001	—^a	—^b	—^b	—^a	U	U	U	U
H1N1	CA04	FJ966082	—^b	R	—^a	U	U	U	U
H5N6	Th001	EPI_ISL_205503	HP	R	—^a	U	U	U	U
H9N2	04.15SZBAXQ005	EPI_ISL_199448	LP	R	—^a	U	U	U	U
Flu-B	Victoria	—^a	—^b	—^b	—^a	U	U	U	U
Flu-B	Yamagata	—^a	—^b	—^b	—^a	U	U	U	U

ISSN: 1471-2334