Fig. 2

Titer evaluation of mouse anti-Omps serum. a Antibody levels in mouse anti-OmpA serum and mouse anti-Smlt4123 serum. Mice were injected with recombinant OmpA or Smlt4123 for three times (5 μg/time), and mice in the control group were injected with the elution buffer (n = 10/group). Two weeks after the final injection, serum were collected. The mouse serum was diluted at 1:102400 and analyzed by ELISA. Values were compared by Student’s t-test. *represents P < 0.05. b IgG1 and IgG2a levels in the pre-immune and post-immune mice injected with recombinant Smlt4123 (n = 8/group). Two weeks after the final immunization with recombinant Smlt4123, sera were collected. The mouse serum was diluted at 1:102400. Values were compared by paired Student’s t-test. * represents P < 0.05