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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Identification of immunogenic outer membrane proteins and evaluation of their protective efficacy against Stenotrophomonas maltophilia

Fig. 1

Two dimensional electrophoresis and Western blot analysis of S. maltophilia Omp preparation. a Image of the 2D gel. S. maltophilia Omps were prepared according to the description in the Methods. A total of 200 μg Omps was analyzed on 2D electrophoresis. After the electrophoresis, the 2D gel was stained with Coomassie Blue. All spots were analyzed by MALDI-TOF-MS and nanoLC-FT ICR MS/MS. Arrows of number 1 to 10 are pointing to the protein spots that had been identified by mass spectrometry. b Image of the Western blot. The 2D gel in (a) was probed with the rabbit anti-S. maltophilia serum. Protein spots 1 and 2 represent OmpA. Protein spots 3 and 4 represent Smlt4123. Protein spot 5 represents Omp TolC. Protein spots 6–9 represent TonB dependent receptor protein. Protein spot 10 represents fatty acid transport system membrane protein

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BMC Infectious Diseases

ISSN: 1471-2334

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