Delayed and highly specific antibody response to nonstructural protein 1 (NS1) revealed during natural human ZIKV infection by NS1-based capture ELISA

Table 1 Specificity and genetic characteristics of mAbs isolated from two ZIKV infected individuals

mAb ID	Origin	Day	Binding to NS1 (EC₅₀)(ng/mL)					Heavy chain			Light chain
mAb ID	Origin	Day	ZIKV	DENV1	DENV2	DENV3	DENV4	V family	CDR3 sequence	V gene identity (%)	V family	CDR3 sequence	V gene identity (%)
ZKns3G2	Pt1	188	34.5	N/A	N/A	N/A	N/A	IGHV3–53	ARERGWLDY	93.68	IGKV1–39	QQTYTIPRT	92.83
ZKns4F10	Pt1	188	10.9	N/A	116.1	N/A	351.4	IGHV4–31	ARAIDNFYDNSI	96.56	IGKV1–39	QQSYSPPYT	94.98
ZKns2E11	Pt2	66	9.8	N/A	N/A	N/A	N/A	IGHV3–30-3	ARVFNGYEGDY	95.83	IGLV3–10	YSTDSSGNLYV	97.49
ZKns14G5	Pt2	66	277.4	N/A	N/A	N/A	N/A	IGHV5–51	ARSNVDGSTDY	98.61	IGLV3–25	QSADSSDTYVPYV	98.92
ZKns4B8	Pt2	66	39.2	N/A	N/A	N/A	N/A	IGHV3–53	ASLGSGSAFGY	96.84	IGKV1–12	QQANSFPFT	98.21

ISSN: 1471-2334