Molecular characterization of mutations associated with resistance to second-line tuberculosis drug among multidrug-resistant tuberculosis patients from high prevalence tuberculosis city in Morocco

Table 1 Primer sequences and positions used to amplify the relevant genes

ATB drug	Gene	Primer	Sequence	Tm	Length
RIF	rpoB	TR 8	5’-TGCACGTCGCGGACCTCCA-3’	58 °C	157 bp
RIF	rpoB	TR 9	5’-TCGCCGCGATCAAGGAGT-3’	58 °C	157 bp
INH	katG	RTB 59	5’-TGGCCGCGGCGGTCGACATT-3’	62 °C	419 bp
	katG	RTB 36	5’-GGTCAGTGGCCAGCATCGTC-3’	62 °C	419 bp
	inhA	inhA P5	5’-CGCAGCCAGGGCCTCGCTG-3’	60 °C	246 bp
	inhA	inhA P3	5’-CTCCGGTAACCAGGACTGA-3’	60 °C	246 bp
FQs	gyrA	gyrA F	5’-TGACATCGAGCAGGAGATGC-3’	59 °C	320 bp
	gyrA	gyrA R	5’-GGGCTTCGGTGTACCTCATC-3’	59 °C	320 bp
	gyrB	gyrB F	5’-GTGGAAATATGTTGGCCGTC-3’	58 °C	413 bp
	gyrB	gyrB R	5’-GTCGTTGTGAACAACGCTGTG-3’	58 °C	413 bp
Aminoglycosides /cyclic peptides	rrs	rrs F	5’-GTAATCGCAGATCAGCAACG-3’	58 °C	216 bp
	rrs	rrs R	5’-GTGATCCAGCCGCACCTT-3’	58 °C	216 bp
	tlyA	tlyA F	5’-GTCTCTGGCCGAACTCGAAG-3’	52 °C	1000 bp
	tlyA	tlyA R	5’-ATTGTCGCCCAATACTTTTTCTAC-3’	52 °C	1000 bp
	eis	eis F	5′-AAA TTC GTC GCT GAT TCT CG-3’	56 °C	387 bp
	eis	eis R	5’-CGC GAC GAA ACT GAG ACC-3’	56 °C	387 bp

ISSN: 1471-2334