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Table 1 Primer sequences and positions used to amplify the relevant genes

From: Molecular characterization of mutations associated with resistance to second-line tuberculosis drug among multidrug-resistant tuberculosis patients from high prevalence tuberculosis city in Morocco

ATB drug Gene Primer Sequence Tm Length
RIF rpoB TR 8 5’-TGCACGTCGCGGACCTCCA-3’ 58 °C 157 bp
TR 9 5’-TCGCCGCGATCAAGGAGT-3’
INH katG RTB 59 5’-TGGCCGCGGCGGTCGACATT-3’ 62 °C 419 bp
RTB 36 5’-GGTCAGTGGCCAGCATCGTC-3’
inhA inhA P5 5’-CGCAGCCAGGGCCTCGCTG-3’ 60 °C 246 bp
inhA P3 5’-CTCCGGTAACCAGGACTGA-3’
FQs gyrA gyrA F 5’-TGACATCGAGCAGGAGATGC-3’ 59 °C 320 bp
gyrA R 5’-GGGCTTCGGTGTACCTCATC-3’
gyrB gyrB F 5’-GTGGAAATATGTTGGCCGTC-3’ 58 °C 413 bp
gyrB R 5’-GTCGTTGTGAACAACGCTGTG-3’
Aminoglycosides
/cyclic peptides
rrs rrs F 5’-GTAATCGCAGATCAGCAACG-3’ 58 °C 216 bp
rrs R 5’-GTGATCCAGCCGCACCTT-3’
tlyA tlyA F 5’-GTCTCTGGCCGAACTCGAAG-3’ 52 °C 1000 bp
tlyA R 5’-ATTGTCGCCCAATACTTTTTCTAC-3’
eis eis F 5′-AAA TTC GTC GCT GAT TCT CG-3’ 56 °C 387 bp
eis R 5’-CGC GAC GAA ACT GAG ACC-3’
  1. ATB Anti-tuberculosis, F forward, R reverse, bp base pair