Table 2 Molecular characteristics of the 14 mcr-1-positive E. coli strains

1

442 (Achtman) Unknown (Pasteur)

IncI1

cma, iroN, iss, lpfA

aadA1, aadA2, bla _TEM-1B , dfrA12, mcr-1 , QnrS1, sul3, tet(A),

2

Unknown (Achtman) 201 (Pasteur)

IncX4, IncP1

astA, iss, lpfA, mchF, tsh

aadA1, aadA2, dfrA12, mcr-1 (‘R’ at nt. 1263), sul3, tet(A)

3

88 (Achtman) 66 (Pasteur)

Not detected

cma, iroN, iss, lpfA

aadA5, mcr-1 , mph(A), strA, strB, sul1

4

Unknown (Achtman) Unknown (Pasteur)

IncX4

iss

aadA1, aadA2, bla _TEM-1B , mcr-1 (27C>T), sul3, tet(B)

5

Unknown (Achtman) 1716 (Pasteur)

IncI1

astA

aac(6’)Ib-cr, aadA5, ARR-3, dfrA17, bla _OXA-1 , mcr-1 , oqxA, oqxB, QnrS2

6

155 (Achtman) 21 (Pasteur)

IncN

lpfA

aadA1, aadA2, aph(3’)-Ia, bla _TEM-1B , dfrA12, mcr-1 , mph(A), oqxA, oqxB, sul2, sul3

7

10 (Achtman) 2 (Pasteur)

IncN

Not detected

aadA1, aadA2, aph(3’)-Ia, bla _TEM-1B , dfrA12, mcr-1 , mph(A), sul2, sul3

8

34 (Achtman) 638 (Pasteur)

Not detected

Not detected

mcr-1

9

226 (Achtman) 486 (Pasteur)

IncR, IncX4

Not detected

mcr-1 , strA, strB, tet(B)

10

5995 (Achtman) Unknown (Pasteur)

IncQ1

Not detected

aph(3’)-Ia, bla _TEM-1B , mcr-1 , strA, strB

11

95 (Achtman) 1 (Pasteur)

IncI1, IncI2, IncQ1

iss, sfaS

aph(3’)-Ia, bla _TEM-1B , dfrA17, mcr-1 , oqxA, oqxB, strA, strB, sul2, tet(B)

12

48 (Achtman) Unknown (Pasteur)

Not detected

Not detected

aadA2, bla_CTX-M-9-like gene, dfrA12, mcr-1, sul3

13

48 (Achtman) Unknown (Pasteur)

IncN, IncHI2

Not detected

aadA1, bla_CTX-M-9-like gene, dfrA12, fosA3, mcr-1, QnrS1, sul1, sul3

14

746 (Achtman) Unknown (Pasteur)

Not detected

astA

aadA1, aadA2, aph(3’)-Ia, dfrA12, mcr-1 , oqxA, oqxB, QnrS1, sul3