Fig. 1

a. Schematic diagram of the bench top model simulating adult tracheal intubation based on a cuffed ETT (TaperGuard Oral Tracheal Tube Evac Murphy Eye, Mallinckrodt ™). b. Laboratory procedure. ALT with SDD or SLT were applied for 2 h during a single dose or a 5-day therapy at the subglottic space after the formation of a 72 h-mature biofilm of P. aeruginosa. After lock therapy, the subglottic area of the ETT was cut into 3 0.5 cm-segments for analysis