|
Specimen type
|
RT-iiPCR
|
CDC-PAHO RT-qPCRa
|
Agreement (k, CI95%)b
|
|---|
| | |
Positive
|
Negative
|
Total
|
|---|
|
Overall performance
|
Positive
|
288
|
38
|
326
|
92% (0.83 [0.77–0.88])
|
| |
Negative
|
1
|
154
|
155
|
| |
Total
|
289
|
192
|
481
|
|
Whole blood
|
Positive
|
60
|
11
|
71
|
90% (0.80 [0.69–0.91])
|
| |
Negative
|
0
|
41
|
41
|
| |
Total
|
60
|
52
|
112
|
|
Plasma
|
Positive
|
74
|
9
|
83
|
92% (0.82 [0.71–0.93])
|
| |
Negative
|
0
|
32
|
32
|
| |
Total
|
74
|
41
|
115
|
|
Serum
|
Positive
|
78
|
6
|
84
|
95% (0.86 [0.76–0.97])
|
| |
Negative
|
0
|
27
|
27
|
| |
Total
|
78
|
33
|
111
|
|
Semen
|
Positive
|
15
|
2
|
17
|
92% (0.81 [0.57–1])
|
| |
Negative
|
0
|
7
|
7
|
| |
Total
|
15
|
9
|
24
|
|
Urine
|
Positive
|
56
|
10
|
66
|
90% (0.79 [0.67–0.89])
|
| |
Negative
|
1
|
45
|
46
|
| |
Total
|
57
|
55
|
112
|
|
Mosquito pools
|
Positive
|
5
|
0
|
5
|
100% (1)
|
| |
Negative
|
0
|
2
|
2
|
| |
Total
|
5
|
2
|
7
|
-
aSamples that yielded inconclusive results (Ct > 38.5) were not included in the analysis. bThe Kappa statistic and 95% confidence interval is shown within brackets
