Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

Table 7 Agreement between the RT-iiPCR and RT-qPCR assays for detection of ZIKV RNA in diverse spiked specimens

Specimen type	RT-iiPCR	CDC-PAHO RT-qPCR^a			Agreement (k, CI95%)^b
		Positive	Negative	Total	Agreement (k, CI95%)^b
Overall performance	Positive	288	38	326	92% (0.83 [0.77–0.88])
	Negative	1	154	155
	Total	289	192	481
Whole blood	Positive	60	11	71	90% (0.80 [0.69–0.91])
	Negative	0	41	41
	Total	60	52	112
Plasma	Positive	74	9	83	92% (0.82 [0.71–0.93])
	Negative	0	32	32
	Total	74	41	115
Serum	Positive	78	6	84	95% (0.86 [0.76–0.97])
	Negative	0	27	27
	Total	78	33	111
Semen	Positive	15	2	17	92% (0.81 [0.57–1])
	Negative	0	7	7
	Total	15	9	24
Urine	Positive	56	10	66	90% (0.79 [0.67–0.89])
	Negative	1	45	46
	Total	57	55	112
Mosquito pools	Positive	5	0	5	100% (1)
	Negative	0	2	2
	Total	5	2	7

^aSamples that yielded inconclusive results (Ct > 38.5) were not included in the analysis. ^bThe Kappa statistic and 95% confidence interval is shown within brackets