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Table 7 Agreement between the RT-iiPCR and RT-qPCR assays for detection of ZIKV RNA in diverse spiked specimens

From: Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

Specimen type RT-iiPCR CDC-PAHO RT-qPCRa Agreement (k, CI95%)b
   Positive Negative Total
Overall performance Positive 288 38 326 92% (0.83 [0.77–0.88])
  Negative 1 154 155
  Total 289 192 481
Whole blood Positive 60 11 71 90% (0.80 [0.69–0.91])
  Negative 0 41 41
  Total 60 52 112
Plasma Positive 74 9 83 92% (0.82 [0.71–0.93])
  Negative 0 32 32
  Total 74 41 115
Serum Positive 78 6 84 95% (0.86 [0.76–0.97])
  Negative 0 27 27
  Total 78 33 111
Semen Positive 15 2 17 92% (0.81 [0.57–1])
  Negative 0 7 7
  Total 15 9 24
Urine Positive 56 10 66 90% (0.79 [0.67–0.89])
  Negative 1 45 46
  Total 57 55 112
Mosquito pools Positive 5 0 5 100% (1)
  Negative 0 2 2
  Total 5 2 7
  1. aSamples that yielded inconclusive results (Ct > 38.5) were not included in the analysis. bThe Kappa statistic and 95% confidence interval is shown within brackets