Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

Table 2 Primer and probe sequences used in the CDC and PAHO RT-qPCR assays

Name	Sequence (5′ to 3′)	Target	Position^a	Function	Reference
ZIKV914prM	TTGGTCATGATACTGCTGATTGC	prM	nt914–936	RT-qPCR forward primer	Lanciotti et al. [17]
ZIKV990prMc	CCTTCCACAAAGTCCCTATTGC	prM	nt990–969	RT-qPCR reverse primer	Lanciotti et al., [17]
ZIKV965prMFAM	FAM-CGGCATACAGCATCAGGTGCATAGGAG-TAMRA	prM	nt939–965	RT-qPCR forward probe	Lanciotti et al., [17]
ZIKV1165E	CCGCTGCCCAACACAAG	E	nt1165–1181	RT-qPCR forward primer	Lanciottiet al., [17]
ZIKV1241Ec	CCACTAACGTTCTTTTGCAGACAT	E	nt1241–1218	RT-qPCR reverse primer	Lanciotti et al., [17]
ZIKV1216HEX	HEX-AGCCTACCTTGACAAGCARTCAGACACTCAA-BHQ1	E	nt1186–1216	RT-qPCR forward probe	Lanciotti et al., [17]
ZIKV4513NS2B	CTGTGGCATGAACCCAATAG	NS2B	nt4513–4532	RT-qPCR forward primer	Waggoner and Pinsky, [43]
ZIKV4603NS2Bc	ATCCCATAGAGCACCACTCC	NS2B	nt4603–4584	RT-qPCR reverse primer	Waggoner and Pinsky, [43]
ZIKV4558cFAM	FAM-CCACGCTCCAGCTGCAAAGG-TAMRA	NS2B	nt4558–4539	RT-qPCR probe	Waggoner and Pinsky, [43]

^aNucleotide position is based on ZIKV PRVABC59 strain (Puerto Rico, 2015), GenBank accession number KX087101.2

ISSN: 1471-2334