Fig. 1
pVXVR-mIFN-γ administration alters the parasite-specific immune response elicited by infection with attenuated parasites. Mice (n = 4) were infected (4 weeks apart) with 3 doses of 105 metacyclic trypomatigotes of the attenuated TCC strain, 50 μg of plasmid pVXVR-mIFN-γ or a combination of both. After each infection dose, serum samples were collected for IgG and cytokines determination. a T. cruzi specific total IgGs levels; b serum levels of parasite specific IgG subtypes, c IL-10 measurement determined by ELISA and (d) IL-10 and IFN-γ levels in supernatant from T. cruzi stimulated spleen cells. Data are representative of three independent experiments. * p < 0.05 Kruskal-Wallis test for single-day measurements with Dunn’s Multiple Comparison Test