Outbreak of caliciviruses in the Singapore military, 2015

BMC Infectious Diseases

Table 1 The list of primers used for genotyping NoV and SaV

		Type	Primer	Sequence (5′ to 3′)	nt position
1	Norovirus GI	Single PCR	G1SKF	CTGCCCGAATTYGTAAATGA	5342^a
1	Norovirus GI	Single PCR	G1SKR	CCAACCCARCCATTRTACA	5671 ^a
2	Norovirus GII	Single PCR	G2SKF	CNTGGGAGGGCGATCGCAA	5058 ^b
2	Norovirus GII	Single PCR	G2SKR	CCRCCNGCATRHCCRTTRTACAT	5401 ^b
3	Sapovirus	1st round PCR	SV-F13	GAYYWGGCYCTCGCYACCTAC	5074 ^c
		1st round PCR	SV-R13	GGTGANAYNCCATTKTCCAT	5876 ^c
		2nd round PCR	SV-F22	SMWAWTAGTGTTTGARATG	5154 ^c
		2nd round PCR	SV-R2	GWGGGRTCAACMCCWGGTGG	5591 ^c

^aNucleotide position of Norwalk virus (GenBank M87661)
^bNucleotide position of Lordsdale virus (GenBank X86557)
^cNucleotide position of Manchester virus (GenBank X86560)
H = A or C or T; K = G or T; M = A or C; N = A or C or G or T; R = A or G; S = C or G; W = A or T; Y = C or T

ISSN: 1471-2334