Fig. 2

a Alignment of the mtDNA cox2 (629 bp) sequences obtained from two larval nematodes removed from CC1 (a and b) and from one larva of the CC2, with respect to the sequences previously obtained from a clinical case due to A. pegreffii (Genbank accession number JQ900760). The nematode larvae from CC1 and CC2 were identified as Anisakis pegreffii, with identity of 99%. The alignment was performed using BioEdit [41]. Dots indicate bps identity. b Alignment of EF1 α-1 nDNA (409 bp) sequences of CC1 (a and b) of two larval nematodes removed from CC1 (a and b) and from one larva from intestine of CC2, with respect to the sequences previously obtained of A. pegreffii (Genbank accession number KT825684) [19]. The nematode larvae from CC1 (a and b) and CC2 were identified as Anisakis pegreffii, with identity of 100 and 99%, respectively. According to the nucleotide diagnostic positions i.e. showing a T and a C in A. pegreffii, respectively, at position 186, and 286, the nematode larvae from the CC1 and CC2 were identified as Anisakis pegreffii