Fig. 2

Identification of TgSOD expression in vitro by fluorescence microscopic detection and Western blotting. Fluorescence microscopy images of TgSOD protein in (a) HEK 293 T cells that were transfected with pEGFP-SOD and (b) empty plasmid pEGFP-C1, and (c) non-transfected HEK 293 T cells; (d) Western blotting of pEGFP-SOD expressed in HEK 293 T cells (lane 1) probed with anti-STAg mouse sera as primary antibody and the protein of SOD is 23 KDa, whereas no band in the negative control cells with the empty plasmid pEGFP-C1 (lane 2) and GAPDH serves as a loading control