Table 1 Reverse transcription polymerase chain reaction (RT-PCR) master mix components for a single reaction carried out using AgPath-ID one–step RT-PCR kit from Applied Biosystems to detect RUBV in FTA cards
From: Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women
No | Component | Volume (mL) |
|---|---|---|
1 | 2 X RT-PCR Buffer | 12▪5 |
2 | 10 μM RV8633 or RV9112 | 1 |
3 | 10 μM RV8945 or RV9577 | 1 |
4 | Nuclease-free water | 9 |
5 | 25XRT-PCR Enzyme Mix | 0.5 |
6 | Extracted DNA template | 1 |
Total volume per reaction | 25▪0 |