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Table 1 Reverse transcription polymerase chain reaction (RT-PCR) master mix components for a single reaction carried out using AgPath-ID one–step RT-PCR kit from Applied Biosystems to detect RUBV in FTA cards

From: Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women

No Component Volume (mL)
1 2 X RT-PCR Buffer 12▪5
2 10 μM RV8633 or RV9112 1
3 10 μM RV8945 or RV9577 1
4 Nuclease-free water 9
5 25XRT-PCR Enzyme Mix 0.5
6 Extracted DNA template 1
  Total volume per reaction 25▪0