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Table 2 Analytical sensitivity and average detection time of RT-SIBA compared with those of RT-PCR for the detection of RSV

From: Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA®

 

RT-SIBA

Average amount of time taken to achieve positive results (minutes)

RT-PCR

Average cycle threshold to achieve positive results (ct)

RSV

RNA copy number per reaction

RSV-A

RSV-B

RSV-A

RSV-B

104

11

13

21

20

103

12

14

24

23

102

14

16

27

26

101

16

20

31

30

0

ND

ND

ND

ND

  1. ND not determined, RSV respiratory syncytial virus, RT-PCR reverse transcription polymerase chain reaction, RT-SIBA reverse transcription strand invasion based amplification