Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA®

BMC Infectious Diseases

Table 1 RT-SIBA oligonucleotides used in this study

Name	Sequence 5′ -- > 3′
RSV-F primer	GAGCCACCTCTCCCAT
RSV-R primer	AGTCAACATTGAGATA
RSV-A IO	CCCCC TTTCTTTTAGCATTTTTTTGTAGGATTT mUmCmUmAmG mAmUmUmCmUmA
RSV-B IO	CCCCC TCTCTTTTAGCATTTTTTTGTAGGACTT mUmCmUmAmG mAmUmUmCmUmA
RSV probe	/56-ROXN/+CA + A + TA + T + T + GA + GA + TA/3IABkFQ/
MS2-F primer	CATGATATTCTGGGCAATAGT
MS2-R primer	CGTAGATGCCTATGGTTC
MS2 IO	CCCCCCCCCC AATAGTCAAATCGACCCAAATCCATTTT mGmGmUmAmA mCmGmCmCmGmGmAmAmC
MS2 probe	/5CY5/A + TA + TT + CT + GG + GC+ A + A/3IAbRQSp/

For invasion oligonucleotides (IOs), bold sequences denote non-homologous seeding area sequences. mA, mC, mG, and mU denote 2′-O-methyl RNA nucleotides. F forward, R reverse, + locked nucleic acid bases, IABkFQ Iowa Black FQ quencher, IAbRQSp Iowa Black RQ quencher, RSV respiratory syncytial virus, RT-SIBA reverse transcription strand invasion based amplification. The RT-SIBA RSV assay was compared with a previously published reverse transcription polymerase chain reaction RSV assay (12)

ISSN: 1471-2334