Table 1 List of primer sequences used for RT-PCR analysis
Gene | FORWARD PRIMER | REVERSE PRIMER |
|---|---|---|
IRAK 1 | 5'-ACCGCAGATTATCATCAACC-3' | 5'-AGACTTACAGCCATACTTCACT-3' |
IRAK 4 | 5'-GCTGTATGTAGGGTGGAAAC-3’ | 5'-TGCTGACAACTGGAAGGTAG-3' |
TRAF6 | 5'-GCCCAGGCTGTTCATAGTTT-3' | 5'-CAAGGGAGGTGGCTGTCATA-3' |
p53 | 5’-CCCAAGCAATGGATGATTTGA-3’ | 5’-GGCATTCTGGGAGCTTCATCT-3’ |
CYCLIN D1 | 5’-AGCTCCTGTGCTGCGAAGTGGAAAC-3’ | 5’-AGTGTTCAATGAAATCGTGCGGGGT-3’ |
CYCLIN E | 5’-CAGCACTTTCT TG AGCAACACCCTC-3’ | 5’-TCTCTAT GTCGCACCACTGATACCC-3’ |
CYCLIN B1 | 5’-AAGAGCTTTAAACTTTGGTCTGGG-3’ | 5’-CTTTGTAAGTCCTTGATTTACCATG-3’ |
CYCLIN A | 5’GCATGTCACCGTTCCTCCTT-3’ | 5’CAGGGCATCTTCACGCTCTAT-3’ |
JNK | 5'- GTACTTGTATGAAACCACCTTTCT -3' | 5'- AGCATCTCTTTCTGAATCTATGAAG -3' |
PI3KCA | 5'- AAGGGTGCTAAAGAGGAACAC -3’ | 5'-CATGAGGTACTGGCCAAAGAT -3' |
PI3KCB | 5'- CTCCAAATGTTGCGCTTGATG -3' | 5'- ACAACTTCAATGAGGCCAGAG -3' |
PI3KCG | 5’- CACCGAGACAGGAAACCTATTT -3’ | 5’- TAGCACAAATGGCACTCTCTC -3’ |
NF-КB | 5’-CGCATCCAGACCAACAACA-3’ | 5’- TGCCAGAGTTTCGGTTCAC-3’ |
p38 | 5’- TCTGCTTACCCTTCACCTTTG -3’ | 5’- CACATCCTCACTCTGCTAGAAAT -3’ |
c-jun | 5’-CAAAGTTTGGATTGCATCAATG-3’ | 5’- TAACATTATAAATGGTCACAGCACATG-3’ |
c-myc | 5’-CTTCTCTCCGTCCTCGGATTCT-3’ | 5’-GAAGGTGATCCAGACTCTGACCTT-3’ |
Sap-1 | 5’-GCTTTTGCCACCACACCACCCATTTCG-3’ | 5’-GCCCAGACAGAGTGAATGGCCCATGAC-3’ |
Elk-1 | 5’-ACCTGAAATCGGAAGAGCTTAAT-3’ | 5’-AACTTCCAACTCTTCCTTGGG-3’ |
TNF-α | 5’-ATGGGCTACAGGCTTGTCACT-3’ | 5’-CTCTTGGCAGCCTTCCTGATT-3’ |
IFN-β | 5’-GTCTCCTCCAAATTGCTCTC-3’ | 5’-ACAGGAGCTTCTGACACTGA-3’ |
IFN-α | 5’-TGGCTGTGAAGAAATACTTCCG-3’ | 5’-TGTTTTCATGTTGGACCAGATG-3’ |
IL-6 | 5’-ATGTAGCCGCCCCACACAGA-3’ | 5’-CATCCATCTTTTTCAGCCAT-3’ |
IL-1β | 5’-ACAGATGAAGTGCTCCTTCCA-3’ | 5’-GTCGGAGATTCGTAGCTGGAT-3’ |
GAPDH | 5'-AAGGCTGTGGGCAAGG-3' | 5'-TGGAGGAGTGGGTGTCG-3' |