Table 1 Primers used for SYBR Green mediated, multiplex real-time PCR assay [10]
Organism | Target region | Primer sequence (5’–3’) | Reaction |
|---|---|---|---|
Mycobacterium avium | 16S | F:CCTCAAGACGCATGTCTTC | I |
R: ACCTACCGTCAATCCGAGAA | |||
Mycobacterium intracelulare | 16S | F:GACCTTTAGRCGCATGTCTTT | I |
R: ACCTACCGTCAATCCGAGAA | |||
Mycobacterium tuberculosis Complex | ITS | F: GCGAGAGCCGGGTGCATG | I |
R: AACAGTGTGTTGGTGGCCAA | |||
AFB genus | 16S | F: CCGCAAGRCTAAAACTCAAA | I/II |
R: TGCACACAGGCCACAAGGGA | |||
MCAG | ITS | F: TAAGGAGCACCATTTCCCAG | II |
R: CGACGTTTTGCCGACTACC | |||
MFG | 16S | F: CCACGCGCTTCATGGTGT | II |
F: CCGCGCTCTTCATGGGGT | |||
F: ACCACGCATTTCATGGTGT | |||
R:ACTTGCGCTTCGTCCCTAT |