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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: Monocytes and B cells support active replication of Chandipura virus

Fig. 3

Detection of transcriptive and replicative form of CHPV in human PBMC subsets. The PBMC was isolated from the whole blood cells healthy donors. CD3+,CD14+,CD19+ cells were separated from PBMCs by MACS separation method and were infected with CHPV at MOI = 5 in complete medium (RPMI + 10 % FCS). Cells were harvested at 24 h pi and total RNA was separated by Trizol method. The transcriptive form (N gene) and replicative form (NP gene) was detected by RT-PCR. The assay was performed in triplicates. Cell control is represented as C in the figure

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