Fig. 6
From: Abolishing HIV-1 infectivity using a polypurine tract-specific G-quadruplex-forming oligonucleotide
ODN A does not accelerate amyloid fibril formation and maintains its antiviral activity in the presence of human semen and SEVI. a PAP248-286 peptides were incubated at 37 °C for 72 h in the presence or absence of 0.5 or 5 μM ODN A. Afterwards, amyloid fibrils were detected by negative-stain transmission electron microscopy. Scale bars indicate 5 μm, 500 nm or 100 nm, left to right. b HIV-1 particles (5 × 108) were incubated for 6 h ± 250 nM ODNs and ± 100 μg/ml SEVI. Jurkat 1G5 T cells were infected overnight with the respective mixtures and HIV-1 p24 antigen release into the supernatants was monitored by ELISA at the indicated days. The p24 antigen level on day 7 post infection is depicted as a bar chart on the right. Two-way ANOVA followed by Bonferroni posttest was used for statistical evaluation. ODN A-mediated inhibition (+/- SEVI; as compared to buffer alone) was highly significant (p < 0.001) at day 14 post infection. c Analysis of HIV-1 infectivity essentially as described in panel b. Prior to de novo infection, HIV-1 particles were incubated for 6 h ± 250 nM ODNs and 2 different human semen samples. Statistics were evaluated as before. ODN A-mediated inhibition (+/- SE1 or SE2; as compared to buffer alone) was highly significant (p < 0.001) at day 10 post infection