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Table 1 Ct value of spiked E. coli dilution series upon in-house human DNA removal step

From: Enrichment of bacterial DNA for the diagnosis of blood stream infections

E. coli density (CFU/ml)

1000

100

10

1

0

MCLB1 processed DNA

29.2 ± 0.5

34 ± 0.5

37 ± 1

Undetectable(a)

Undetectable(a)

NaOH/SDS processed DNA

29.0 ± 0.5

37 ± 1

Undetectable(a)

Undetectable(a)

Undetectable(a)

  1. (a)Undetectable means that the real-time PCR fluorescent signal appears later than that of cut-off assays (see the detection limits and negative control validation)