The effect of growth rate on pyrazinamide activity in Mycobacterium tuberculosis - insights for early bactericidal activity?

Pullan, Steven T.; Allnutt, Jon C.; Devine, Rebecca; Hatch, Kim A.; Jeeves, Rose E.; Hendon-Dunn, Charlotte L.; Marsh, Philip D.; Bacon, Joanna

doi:10.1186/s12879-016-1533-z

Table 2 Ribosomal and tRNA associated genes down-regulated in M. tuberculosis exposed to PZA under slow growth

From: The effect of growth rate on pyrazinamide activity in Mycobacterium tuberculosis - insights for early bactericidal activity?

Gene expression fold change from pre-PZA to early recovery phase (100 μgml⁻¹ for 4 h)	Gene expression fold change from pre-PZA to iv) late recovery phase (100 μgml⁻¹ for 2 MGT)	Gene name	UniProt ID	Annotation
−4.81	−11.22	tRNA-Val		tRNA-Val
−3.23	−10.01	tRNA-Glu		tRNA-Glu
−3.99	−9.34	tRNA-Lys		tRNA-Lys
−5.13	−8.95	Rv0700	P0A5X0	30S ribosomal protein S10
−2.63	−8.62	tRNA-Gly		tRNA-Gly
−3.52	−8.60	tRNA-Thr		tRNA-Thr
−4.86	−8.15	tRNA-Arg		tRNA-Arg
−5.22	−7.36	Rv0704	P95052	50S ribosomal protein L2
−4.49	−7.33	tRNA-Met		tRNA-Met
−4.68	−6.35	Rv0703	P95051	50S ribosomal protein L23
−4.29	−6.16	Rv1298	P66187	50S ribosomal protein L31
−1.65	−6.13	tRNA-Leu		tRNA-Leu
−2.33	−5.97	tRNA-Ser		tRNA-Ser
−4.08	−5.88	Rv0710	P95058	30S ribosomal protein S17
−1.65	−5.43	tRNA-Pro		tRNA-Pro
−3.70	−5.20	Rv0705	P0A5X4	30S ribosomal protein S19
−1.44	−4.83	tRNA-Tyr		tRNA-Tyr
−3.56	−4.82	Rv0709	P95057	50S ribosomal protein L29
−4.32	−4.82	Rv0702	P60729	50S ribosomal protein L4
−3.99	−4.72	Rv0722	P66181	50S ribosomal protein L30
−3.09	−4.66	Rv0701	P60442	50S ribosomal protein L3
−3.76	−4.17	Rv0716	P62403	50S ribosomal protein L5
−3.32	−3.98	Rv0717	P0A5X2	30S ribosomal protein S14 type Z
−3.48	−3.79	Rv0682	P41196	30S ribosomal protein S12
−3.01	−3.69	Rv0715	P60627	50S ribosomal protein L24
1.03	−3.68	tRNA-Cys		tRNA-Cys
−3.45	−3.61	Rv1643	P66105	50S ribosomal protein L20
−2.99	−3.56	Rv0723	P95071	50S ribosomal protein L15
−1.44	−3.38	tRNA-Trp		tRNA-Trp
−2.97	−3.32	Rv2412	P66505	30S ribosomal protein S20
−3.07	−3.22	Rv0683	P41194	30S ribosomal protein S7
−4.07	−3.05	Rv0718	P66625	30S ribosomal protein S8
−3.13	−3.04	Rv1642	P66271	50S ribosomal protein L35
−2.59	−3.02	Rv0714	P66069	50S ribosomal protein L14
−2.79	−2.92	Rv0707	P0A5X6	30S ribosomal protein S3
−1.06	−2.91	5S_rRNA		ribosomal RNA
−2.44	−2.91	Rv0721	P66574	30S ribosomal protein S5
1.03	−2.84	tRNA-Asp		tRNA-Asp
−1.14	−2.67	tRNA-His		tRNA-His
−1.10	−2.65	tRNA-Phe		tRNA-Phe
−1.45	−2.64	tRNA-Ile		tRNA-Ile
1.00	−2.45	23S rRNA		ribosomal RNA
−1.41	−2.39	Rv0640	P66056	50S ribosomal protein L11
1.00	−2.27	16S rRNA		Ribosomal RNA
−2.01	−2.27	Rv0720	P66076	50S ribosomal protein L18
−1.84	−2.17	Rv0719	P66311	50S ribosomal protein L6
−2.15	−2.07	Rv0055	P69230	30S ribosomal protein S18 1
−1.67	−2.03	Rv1630	O06147	30S ribosomal protein S1
−2.35	−1.92	Rv1644	P94978	possible 23S rRNA methyltransferase tsnR

Genes encoding tRNAs or ribosome component proteins that were down-regulated by at least two fold (with an ANOVA p –value of less than 0.05) in M. tuberculosis under slow growth in the early recovery phase (4 h after the addition of 100 μgml⁻¹ PZA) and/or the late recovery phase (2 MGT after the addition of 100 μgml⁻¹ PZA) compared to the pre-PZA phase

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ISSN: 1471-2334

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