Fig. 2

Patient BI and control brain samples from occipital cortex grey matter and occipital subcortical white matter were analysed using confocal microscopy for presence of C.b. antigens and co-stained for GFAP. Tissues were stained with anti-GFAP primary antibodies/FITC (green)-conjugated secondary antibodies; and co-stained with anti-C.b. primary antibodies/PE-conjugated (red) secondary antibodies as described in Material and Methods. Hoechst (blue) was used to stain cell nuclei. Magnification was set at 400X. a BI’s occipital subcortical white matter; b control patient’s occipital subcortical white matter; c BI’s occipital cortex grey matter; d control patient’s occipital cortex grey matter